电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁到

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简述 ：探讨伽马射线是否可通过转化糖蛋白-β（TGF-β）-介导的视网膜-间质转换 (EMT)来促进巨噬细胞的洪水泛滥搬迁。使用年均2Gy(60)Coγ中央线光照源自人类器官的6种巨噬细胞，记录与EMT相关的变异，这包括分别并用全像系统设计，蛋白质印迹方法，免疫荧光系统设计，划痕试验和Transwell小室试验来观察并样品巨噬细胞组织形态，EMT上标，洪水泛滥搬迁能力等。换用蛋白酶联免疫吸附法样品这些巨噬细胞里TGF-β蛋白水平，并用比如说诱发剂SB431542来评估TGF-β接收机途径在伽马射线EMT里的作用。经过年均为2Gy光照的巨噬细胞里存在间叶巨噬细胞的表达，与骗光照组相比其视网膜上标缩减，间叶巨噬细胞上标增加，同时其洪水泛滥转回能力弱化，TGF-β蛋白水平也提高。进一步发现由A549伽马射线诱导的EMT可通过对TGF-β接收机诱发发生逆转。这些结果表明TGF-β介导的EMT在伽马射线诱导弱化巨噬细胞洪水泛滥转回能力里起着关键作用。

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